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chemi doc xrs imaging software  (Bio-Rad)


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    Structured Review

    Bio-Rad chemi doc xrs imaging software
    Chemi Doc Xrs Imaging Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 34698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chemi+doc+xrs+imaging+software/ChemiDoc+Imaging+System/pmc11440729-115-12-16
    Average 99 stars, based on 34698 article reviews
    chemi doc xrs imaging software - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Over-expression of Sirt1 contributes to chemoresistance and indicates poor prognosis in serous epithelial ovarian cancer (EOC).
    Article Snippet: Ovarian cancer is the most lethal malignancy in female patients, and chemoresistance is the major contribution to low over survival rate.. We aim to investigate the correction between Sirt1 expression and chemoresistance in serous epithelial ovarian cancer (EOC) and prognosis significance of Sirt1.. Immunochemistry was used to determine the location pattern and expression of Sirt1 in a total of 63 serous EOC patients (28 cases of chemoresistance patients and 35 chemosensitive).The relationship between Sirt1 expression and clinicopathological features of serous EOC was analyzed.

    Article Title: The up-regulation of PAK2 indicates unfavorable prognosis in patients with serous epithelial ovarian cancer and contributes to paclitaxel resistance in ovarian cancer cells.
    Article Snippet: Following blocking with 5% skimmed milk, the membranes were incubated with anti-PAK2 (Abcam, Cambridge, UK, 1:500) and anti-GAPDH antibodies (Abcam, Cambridge, UK, 1:1000), and subsequently with HRP-conjugated anti-rabbit secondary antibodies (Abcam, Cambridge, UK, 1:2000). .. The protein bands were visualized using an ECL system and quanti ed using Chemi-Doc XRS imaging software (Bio-Rad). .. Gene expression The total RNAs of all cell groups were isolated using the Trizol reagent (Invitrogen, CA, USA) following the provided protocol.

    Article Title: Over-expression of nuclear NF-κB1 and c-Rel correlates with chemoresistance and prognosis of serous epithelial ovarian cancer.
    Article Snippet: Article history: Received 26 September 2015 and in revised form 29 November 2015 Accepted 30 November 2015 Available online 9 December 2015 Objective: This study aims to measure the expression and subcellular location of NF-κB1 and c-Rel protein in serous epithelial ovarian cancer (EOC), and to test the correlation between NF-κB1 and c-Rel expression and clinicopathological parameters, chemoresistance, and prognosis of serous EOC.. Methods: A total of 63 specimens of serous EOC patients meeting the inclusion criteria with complete follow-up data were enrolled in our study.. The specimens were divided into chemo-resistant group and sensitive group.

    Article Title: Over-expression of ABCC5 indicates poor prognosis of ovarian cancer and contributes to paclitaxel resistance in ovarian cancer cells
    Article Snippet: After blocking with 5% skimmed milk, the membranes were incubated with anti-ABCC5 (Abcam, Cambridge, UK, 1:50), and anti-GAPDH antibodies (Abcam, Cambridge, UK, 1:500), followed by incubation with HRP-conjugated anti-rabbit (Abcam, Cambridge, UK,1:2000). .. Bands were visualized with ECL system and were quanti ed using Chemi-Doc XRS imaging software (Bio-Rad). .. Gene expression of ABCC5 Total RNAs of all the cell group were isolated using the Trizol reagent (Invitrogen, CA, USA) according to the protocol.

    Article Title: Down-regulated expression of miR-134 contributes to paclitaxel resistance in human ovarian cancer cells.
    Article Snippet: Membranes were washed with Tris buffered saline (TBS, Sigma Aldrich, St. Louis, MO, USA), blocked with 5% dried non-fat milk (Bio-Rad Laboratories, Hercules, CA, USA) in 1% Tween–TBS and probed with antibody raised against Pak2 (Abcam, Cambridge, UK, 1:500), Bad (Abcam, 1:500), phospho-Bad (Ser112, Ser136) (CST, MA, USA, 1:1000) with GAPDH (Abcam, 1:2000) as a visual loading control. .. Bands were visualized by secondary antibody IgG-linked horseradish peroxidase conjugate (1:2000) with ECL and were quantified using Chemi-Doc XRS imaging software (Bio-Rad). ..

    Article Title: NF-κB1, c-Rel, and ELK1 inhibit miR-134 expression leading to TAB1 upregulation in paclitaxel-resistant human ovarian cancer
    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with rabbit antibody of NF-κB1, c-Rel, ELK1, rabbit anti-TAB1 antibody (Abcam, 1:500), and mouse anti-GAPDH antibody (Abcam, 1:5000), respectively followed by incubation for 1 h at room temperature with a horseradish peroxidase-conjugated detection antibody (1:2000). .. The immunoreactive bands were visualized with an electrochemiluminescence system and quantified using Chemi-Doc XRS imaging software (Bio-Rad). ..

    Article Title: The up-regulation of PAK2 indicates unfavorable prognosis in patients with serous epithelial ovarian cancer and contributes to paclitaxel resistance in ovarian cancer cells
    Article Snippet: After blocking with 5% skimmed milk, the membranes were subjected to incubation with anti-PAK2 (Abcam, Cambridge, UK, 1:500) and anti-GAPDH antibodies (Abcam, Cambridge, UK, 1:1000), followed by incubation with HRP-conjugated anti-rabbit secondary antibodies (Abcam, Cambridge, UK, 1:2000). .. The protein bands were detected using an ECL system and quantified using Chemi-Doc XRS imaging software (Bio-Rad). .. The total RNA from all cell groups were isolated utilizing Trizol reagent (Invitrogen, CA, USA) according to the provided protocol.

    Software:

    Article Title: Over-expression of Sirt1 contributes to chemoresistance and indicates poor prognosis in serous epithelial ovarian cancer (EOC).
    Article Snippet: Ovarian cancer is the most lethal malignancy in female patients, and chemoresistance is the major contribution to low over survival rate.. We aim to investigate the correction between Sirt1 expression and chemoresistance in serous epithelial ovarian cancer (EOC) and prognosis significance of Sirt1.. Immunochemistry was used to determine the location pattern and expression of Sirt1 in a total of 63 serous EOC patients (28 cases of chemoresistance patients and 35 chemosensitive).The relationship between Sirt1 expression and clinicopathological features of serous EOC was analyzed.

    Article Title: The up-regulation of PAK2 indicates unfavorable prognosis in patients with serous epithelial ovarian cancer and contributes to paclitaxel resistance in ovarian cancer cells.
    Article Snippet: Following blocking with 5% skimmed milk, the membranes were incubated with anti-PAK2 (Abcam, Cambridge, UK, 1:500) and anti-GAPDH antibodies (Abcam, Cambridge, UK, 1:1000), and subsequently with HRP-conjugated anti-rabbit secondary antibodies (Abcam, Cambridge, UK, 1:2000). .. The protein bands were visualized using an ECL system and quanti ed using Chemi-Doc XRS imaging software (Bio-Rad). .. Gene expression The total RNAs of all cell groups were isolated using the Trizol reagent (Invitrogen, CA, USA) following the provided protocol.

    Article Title: Over-expression of nuclear NF-κB1 and c-Rel correlates with chemoresistance and prognosis of serous epithelial ovarian cancer.
    Article Snippet: Article history: Received 26 September 2015 and in revised form 29 November 2015 Accepted 30 November 2015 Available online 9 December 2015 Objective: This study aims to measure the expression and subcellular location of NF-κB1 and c-Rel protein in serous epithelial ovarian cancer (EOC), and to test the correlation between NF-κB1 and c-Rel expression and clinicopathological parameters, chemoresistance, and prognosis of serous EOC.. Methods: A total of 63 specimens of serous EOC patients meeting the inclusion criteria with complete follow-up data were enrolled in our study.. The specimens were divided into chemo-resistant group and sensitive group.

    Article Title: Over-expression of ABCC5 indicates poor prognosis of ovarian cancer and contributes to paclitaxel resistance in ovarian cancer cells
    Article Snippet: After blocking with 5% skimmed milk, the membranes were incubated with anti-ABCC5 (Abcam, Cambridge, UK, 1:50), and anti-GAPDH antibodies (Abcam, Cambridge, UK, 1:500), followed by incubation with HRP-conjugated anti-rabbit (Abcam, Cambridge, UK,1:2000). .. Bands were visualized with ECL system and were quanti ed using Chemi-Doc XRS imaging software (Bio-Rad). .. Gene expression of ABCC5 Total RNAs of all the cell group were isolated using the Trizol reagent (Invitrogen, CA, USA) according to the protocol.

    Article Title: Down-regulated expression of miR-134 contributes to paclitaxel resistance in human ovarian cancer cells.
    Article Snippet: Membranes were washed with Tris buffered saline (TBS, Sigma Aldrich, St. Louis, MO, USA), blocked with 5% dried non-fat milk (Bio-Rad Laboratories, Hercules, CA, USA) in 1% Tween–TBS and probed with antibody raised against Pak2 (Abcam, Cambridge, UK, 1:500), Bad (Abcam, 1:500), phospho-Bad (Ser112, Ser136) (CST, MA, USA, 1:1000) with GAPDH (Abcam, 1:2000) as a visual loading control. .. Bands were visualized by secondary antibody IgG-linked horseradish peroxidase conjugate (1:2000) with ECL and were quantified using Chemi-Doc XRS imaging software (Bio-Rad). ..

    Article Title: NF-κB1, c-Rel, and ELK1 inhibit miR-134 expression leading to TAB1 upregulation in paclitaxel-resistant human ovarian cancer
    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with rabbit antibody of NF-κB1, c-Rel, ELK1, rabbit anti-TAB1 antibody (Abcam, 1:500), and mouse anti-GAPDH antibody (Abcam, 1:5000), respectively followed by incubation for 1 h at room temperature with a horseradish peroxidase-conjugated detection antibody (1:2000). .. The immunoreactive bands were visualized with an electrochemiluminescence system and quantified using Chemi-Doc XRS imaging software (Bio-Rad). ..

    Article Title: The up-regulation of PAK2 indicates unfavorable prognosis in patients with serous epithelial ovarian cancer and contributes to paclitaxel resistance in ovarian cancer cells
    Article Snippet: After blocking with 5% skimmed milk, the membranes were subjected to incubation with anti-PAK2 (Abcam, Cambridge, UK, 1:500) and anti-GAPDH antibodies (Abcam, Cambridge, UK, 1:1000), followed by incubation with HRP-conjugated anti-rabbit secondary antibodies (Abcam, Cambridge, UK, 1:2000). .. The protein bands were detected using an ECL system and quantified using Chemi-Doc XRS imaging software (Bio-Rad). .. The total RNA from all cell groups were isolated utilizing Trizol reagent (Invitrogen, CA, USA) according to the provided protocol.

    Electrochemiluminescence:

    Article Title: NF-κB1, c-Rel, and ELK1 inhibit miR-134 expression leading to TAB1 upregulation in paclitaxel-resistant human ovarian cancer
    Article Snippet: Subsequently, the membranes were incubated overnight at 4°C with rabbit antibody of NF-κB1, c-Rel, ELK1, rabbit anti-TAB1 antibody (Abcam, 1:500), and mouse anti-GAPDH antibody (Abcam, 1:5000), respectively followed by incubation for 1 h at room temperature with a horseradish peroxidase-conjugated detection antibody (1:2000). .. The immunoreactive bands were visualized with an electrochemiluminescence system and quantified using Chemi-Doc XRS imaging software (Bio-Rad). ..



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